水溶性染色剂(PI)能与核酸结合而使细胞核染色,可将其应用于细胞

水溶性染色剂(PI)能与核酸结合而使细胞核染色,可将其应用于细胞死活的鉴别。细胞浸泡于一定浓度的PI中,仅有死亡细胞的核全被染色,活细胞则不着色,但将PI注射到活细胞中,则细胞核会着色,利用PI鉴别细胞死活的基本原理是(  )

A.死细胞与活细胞的核酸结构不同

B.死细胞与活细胞的核酸含量不同

C.活细胞能分解染色剂PI

D.活细胞的细胞膜阻止PI的进入

答案

D

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