以下是有关酶的两个实验,根据表格内容分析回答下列问题。 表1

以下是有关酶的两个实验,根据表格内容分析回答下列问题。

表1

操作步骤

操作方法

试管A

试管B

试管C

1

加淀粉溶液

2 mL

2 mL

2 mL

2

加淀粉酶溶液

1 mL

1 mL

1 mL

3

温度处理

60 ℃

100 ℃

0 ℃

4

加碘液

2滴

2滴

2滴

表2

操作步骤

操作方法

试管A

试管B

1

加淀粉溶液

2 mL

2

加蔗糖溶液

2 mL

3

加斐林试剂甲

2 mL

2 mL

4

加斐林试剂乙

数滴

数滴

5

加淀粉酶溶液

1 mL

1 mL

 

(1)表1为探究 ________的实验。

(2)该实验把温度处理改为pH分别为5、7、9,能否用于探究pH对酶活性的影响,为什么? ______________________________________________________________________________________________                             _________________________________。

(3)表2为探究____________的实验。

(4)请修正表2操作步骤中的错误:________;________;________。

 

答案

(1)酶的活性受温度影响

(2)不能,因为氢氧化钠与碘液反应,不能在碱性条件下用碘液对淀粉进行鉴定

(3)酶的专一性

(4)斐林试剂甲和乙要等量混合后使用 应先加酶溶液后加斐林试剂 加斐林试剂后要水浴加热

 

根据实验题目可以确定自变量,根据自变量也可以确定实验题目:碘液会与氢氧化钠溶液反应,所以在碱性条件下不能用碘液鉴定淀粉;斐林试剂的用法与双缩脲试剂的用法不同,要将斐林试剂甲和乙等量混合后使用,并且要进行水浴加热。

 

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