下列属于PCR技术的条件的是 ①单链的脱氧核苷酸序列引物  ②目的

下列属于PCR技术的条件的是

①单链的脱氧核苷酸序列引物  ②目的基因所在的DNA片段    ③脱氧核苷酸    ④核糖核苷酸    ⑤DNA连接酶     ⑥DNA聚合酶       ⑦DNA限制性内切酶  

A.①②③⑤      B.①②③⑥      C.①②③⑤⑦        D.①②④⑤⑦

答案

B


解析:

PCR技术要求5方面条件:1、模板,本题为②项;2、引物,本题为①项;3、原料,本题为合成DNA,故为③脱氧核苷酸,合成RNA才需要核糖核苷酸;4、酶,本题为⑥DNA聚合酶,而⑤DNA连接酶是用于连接磷酸二酯键,错;⑦DNA限制性内切酶为切割磷酸二酯键,错;5、能量,应为ATP,本题未涉及。故选B项

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