为验证pH对唾液淀粉酶活性的影响,某学生设计了下表中的实验方法

为验证pH对唾液淀粉酶活性的影响,某学生设计了下表中的实验方法步骤。在同学们提出的下列评价中,不合理的是(   )

实验步骤

项目

试管1

试管2

1

加入可溶性淀粉溶液

2 mL

2 mL

2

加入新鲜唾液淀粉酶溶液

1 mL

1 mL

3

 

 

 

加入1 mol·L-1的盐酸溶液

/

1 mL

 

加入1 mol·L-1的NaOH溶液

1 mL

/

 

4

置于37 ℃水浴中

5 min

5 min

5

滴入碘液

2滴

2滴

A.缺少pH为6.8的对照组

B.都置于37 ℃水浴中可排除温度对酶活性的影响

C.检验实验结果的试剂选择是合理的

D.操作步骤设计合理

 

答案

B

A项错误,需水浴加热;C项错误,即使用黄化苗,也应该含有类胡萝卜素;D项错误,两试管中反应速率的差异是由于前者降低活化能的作用更显著。

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