DNA的粗提取与鉴定实验中有三次过滤: ⑴过滤用蒸馏水稀释过的鸡

DNA的粗提取与鉴定实验中有三次过滤:

⑴过滤用蒸馏水稀释过的鸡血细胞液

⑵过滤含粘稠物的0.14mol/LNaCl溶液

⑶过滤溶解有DNA的2mol/LNaCl溶液

以上三次过滤分别为了获得(   )

A含核物质的滤液、纱布上的粘稠物、含DNA的滤液

B含核物质的滤液、滤液中DNA粘稠物、含DNA的滤液

C含核物质的滤液、滤液中DNA粘稠物、纱布上的DNA

D含较纯的DNA滤液、纱布上的粘稠物、含DNA的滤液

答案

A


解析:

用蒸馏水稀释鸡血细胞液,使血细胞的细胞膜、核膜破裂,释放出核物质,此时过滤只能得到含DNA和其他物质如蛋白质的滤液,这种滤液必须经过实验中其他步骤得相继处理,方能得到较纯的DNA。DNA在0.13mol/LNaCl溶液中溶解度最低,成丝状粘稠物,可经过滤留在纱布上。

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